Fig. 3. Thrashing rate of 1d-old Htt51Q(-1) C. elegans after RNAi knockdown. (A) Motility was analyzed by counting the body bends for each nematode within 30 s. Each dot represents one individual, the horizontal bar represents mean ± SEM (n = 10 for qars-1, yars-1, rsks-1, daf-2, daf-16; n = 20 for control RNAi/empty L4440 vector, n = 30 for hsf-1, ifg-1). Data were normalized to the thrashing rate of control animals. ** p<0.01 for ifg-1 compared to the control, differences between all other knockdowns were not significant. (B) Filter-retardation assay of synchronized animals expressing Htt51Q(-1) grown on control RNAi/L4440 vector or nsun-5 RNAi and subjected at 1d of adulthood to various doses of osmotic stress. 30 animals were loaded onto a cellulose-acetate membrane and frameshifted Htt51Q(-1) detergent-insoluble aggregates were detected using anti-mCherry antibodies.